Mushroom Enzyme Lab Results

Now that we have finished the Data Collection part of the enzyme lab it's time to analyze your results! 

1.) Copy the average data tables for each experiment (I have calculated the averages for you- lucky!)

2.)  Create a single graph with all of the data so you can compare the data from all 3 experimental groups and the control

3.)  Answer Post-Lab questions below

The Exact information will not be needed for tomorrows exam.  However, it is important to understand how enzymes work since they are proteins (a major macromolecule) and how temperature, inhibitors and pH may affect them.

You will want to finish this part of your lab before class on FRIDAY. This gives you tonight to concentrate on other test topics.

Post-Lab Questions:
1.  What does color change tell you about enzyme activity?
2.  What differences did you observe between the control and experimental trials?
3.  For your experimental condition (temp, pH, or Inhibitor), explain how that condition affected enzyme activity.
4.  How does the control reaction help you interpret your experimental results?  How would you know if enzyme activity was changed in the experimental reaction if you had no control reaction data?

Answer the following questions about each experimental group

5a.  For the pH experiment did the pH increase or decrease? Why is this?  How did this affect experimental results?

5b.  How was temperature changed?  How did this affect experimental results?

5c.  What was the inhibitor used (stuck?  read the questions further...)?  How did this affect the experimental results?  Why would sodium benzoate be used as a food preservative?